![]() In: Pai SI, Faller AJ, Lincoln TL, Trytten DA, Wilkerson TD (eds) Dynamics of fluids and plasmas. Rhea RP (1966) Microcinematographic, electron microscopic and electrophysiological studies on shuttle streaming in the slime mold Physarum polycephalum. ![]() Revel JP, Karnovsky J (1967) Hexagonal arrays of subunits in intracellular junctions of the mouse heart and liver. Miller DJ, Mörchen A (1978) On the effects of divalent cations and ethylene glycol-bis-(β-amino-ethyl ether) N, N, N′ N′,-tetraacetate on action potential duration in frog heart. Meyer R, Stockem W (1979) Studies on microplasmodia of Physarum polycephalum V: Electrical activity of different types of microplasmodia and macroplasmodia. McLaughlin S, Mulrine N, Gresalfi T, Vaio G, McLaughlin A (1981) Adsorption of divalent cations to bilayer membranes containing phosphatidylserine. McClory A, Coote JG (1985) The chemotactic response of the myxomycete Physarum polycephalum to amino acids, cyclic nucleotides and folic acid. Matveeva NB, Beylina SI, Teplov VA, Layrand DB (1978) Chemotactic and proton responses of the slime mold Physarum polycephalum to non-metabolizable glucose analogues. Fine structural localization in animal tissues. Kuroda H, Kuroda R (1981) Origin of the membrane potential in plasmodial droplets of Physarum polycephalum. Kukulies J, Stockem W, Wohlfarth-Bottermann KE (1983) Caffeine-induced surface blebbing and budding in the acellular slime mold Physarum polycephalum. In: Aldrich HC, Daniel JW (eds) Cell biology of Physarum and Didymium, Academic Press, New York, pp 145–208 Kessler D (1982) Plasmodial structure and motility. Kamiya N, Abé S (1950) Bioelectrical phenomena in the myxomycete plasmodium and their relation to protoplasmatic flow. Hirose T, Ueda T, Kobatake Y (1982) Changes in intracellular pH accompanying chemoreception in the plasmodia of Physarum polycephalum. Hatano S (1970) Specific effect of Ca 2+ on movement of plasmodial fragment obtained by caffeine treatment. Hale CW (1946) Histochemical demonstration of acid polysaccharides in animal tissue. J Gen Microbiol 25: 47–49įingerle J, Gradmann D (1982) Electrical properties of the plasma membrane of microplasmodia of Physarum polycephalum. Bull Torrey Bot Club 63: 205–210ĭaniel JW, Rusch HF (1961) The pure culture of Physarum polycephalum on a partially soluble medium. J Cell Biol 101: 1463–1472Ĭamp WG (1936) A method of cultivating myxomycete plasmodia. J Membrane Biol 94: 83–97īoyles JK, Fox JEB, Phillips DR, Stenberg PE (1985) Organization of the cytoskeleton in resting, discoid platelets: preservation of actin filaments by a modified fixation that prevents osmium damage. The results strongly support the hypothesis that the MP in Physarum cell fragments is mainly generated by an electrogenic H +-extrusion mechanism.īowman BJ, Bowman EJ (1986) H +-ATPases from mitochondria, plasma membranes, and vacuoles of fungal cells. Metabolic inhibition by potassium cyanide or low temperature (11☌) as well as application of the protonophore CCCP caused a depolarization of the MP. The MP was not much affected by changes in external, , or but was sensitive to changes in or, with a linear dependence on pH 0 in the range between 7 and 5. The ionic nature of MP was studied by varying the composition of the perfusing medium. Spontaneous depolarizations of the MP, with amplitudes between 10 and 80 mV and a duration of 20–30 s, failed to show a correlation with contractile activity. Electron microscopical staining with ruthenium red, iron or lanthanum delivered evidence for localization of lectin and calcium binding sites in a thin mucous layer on the cell surface.Įlectrical recordings by means of intracellular microelectrodes yielded an average membrane potential (MP) of −113 mV. In addition, binding sites for external calcium ions were detected by chlorotetracycline-fluorescence. Analysis of cell surface composition with the fluorescence microscope and different RITC-conjugated lectins revealed strong binding of ConA and RCA-I, weak binding of PEA, DBA and WGA and no binding of UEA-I. Small, spherical cell fragments derived from macroplasmodia of the acellular slime mold Physarum polycephalum by incubation in a 15 mM caffeine solution were investigated with morphological and electrophysiological techniques.
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